DNS-Libraries and -Sequencing

Competence of the Z-Project is the coordination of DNS synthesizing and sequencing and the administration of the designated central budget. The coordination of DNS sequencing is supported by the Core Facility Screening and Automation Technologies (SYNMIKRO, UMR).

Staring point of the DNS synthesizing and sequencing is the procurement of oligonucletide libraries. These libraries are used to create double stranded DNS fragments which are then used at Project Area B. The draft of these sequences is created at Area A. Starting with a pool of single stranded oligonucletides, a sorted set of fragments for the modular assembling of data sets for the pilot project is produced in cooperation with the Core Facility Screening and Automation Technologies. The draft of these sequenced fragments, too, is created at Area A. They can be assembled with the MoClo-System81 and consist of a short information encoded core. This core is flanked by variable sequences (to avoid homologue recombinations) and adapters which allow amplification and assembling.

The Core Facility Screening and Automation Technologies has multiple laboratory automation systems, a Illumina MiSeq sequencing device and a bioreactor for continuous cultivation of experimental evolutions. Depending on the required sequencing technology and capacity, sequencing is carried out in house or at external providers.